Molecular taxonomy of hypothalamic cell types
Campbell JN, Macosko EZ, Fenselau H, Pers TH, Lyubetskaya A, Tenen D, Goldman M, Verstegen AM, Resch JM, McCarroll SA, Rosen ED, Lowell BB, Tsai LT. A molecular census of arcuate hypothalamus and median eminence cell types. Nat Neurosci. 2017 Mar;20(3):484-496. PMCID: PMC5323293.
Check a gene’s expression in Arc-ME cell types here
Tao J, Campbell JN, Tsai LT, Wu C, Liberles SD, Lowell BB. Highly selective brain-to-gut communication via genetically defined vagus neurons. Neuron. 2021 Jul 7;109(13):2106-2115.e4. PMC8273126..
Check a gene expression in DMV Chat+ populations here
Single cell profiling of Adipose Tissue
Li J, Li E, Czepielewski R, Chi J, Guo X, Han YH, Wang D, Wang L, Hu B, Dawes B, Jacobs CL, Tenen DE, Lin S, Lee B, Morris D, Tobias A, Randolph G, Cohen P, Tsai LT, Rosen ED. Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells. accepted Cell Metabolism.
Westcott GP, Emont MP, Li J, Jacobs C, Tsai L, Rosen ED. Mesothelial cells are not a source of adipocytes in mice. Cell Rep. 2021 Jul 13;36(2):109388. PMC8317472.
Emont M, Jacobs C, Essene A, Pant D, Tenen D, Colleluori G, Vincenzo A, Jørgensen A, Dashti H, Stefek A, McGonagle E, Strobel S, Laber S, Agrawal S, Westcott G, Kar A, Veregge M, Gulko A, Srinivasan H, Kramer Z, Filippis E, Merkel E, Ducie J, Boyd C, Gourash W., Courcoulas A, Lin S, Lee B, Morris D, Tobias A, Khera A, Claussnitzer M, Pers T, Giordano A, Ashenberg O, Regev A, Tsai L, Rosen E. A single cell atlas of human and mouse white adipose tissue. Nature, 2022,
Check a gene expression in Mouse or Human visceral and subcutaneous adipose tissue here
Gene regulatory networks governing insulin resistance in adipocytes
Tsai L*, Kang S*, Zhou Y, Evertts A, Xu S , Griffin M, Issner R, Whitton H, Garcia B, Epstein C, Mikkelsen T, Rosen E. Identification of nuclear hormone receptor pathways causing insulin resistance by transcriptional and epigenomic analysis. Nature Cell Biology. 2015 Jan;17(1):44-56. PMCID: PMC4281178. (* Both authors contributed equally to this work)
Kang S, Tsai LT, Rosen ED. Nuclear Mechanisms of Insulin Resistance. Trends Cell Biol. Trends Cell Biol. 2016 May;26(5):341-51. PMCID 4844850.
Roh HC, Kumari M, Taleb S, Tenen D, Jacobs C, Lyubetskaya A, Tsai LT, Rosen ED. Adipocytes fail to maintain cellular identity during obesity due to reduced PPARγ activity and elevated TGFβ-SMAD signaling. Mol Metab. 2020 Dec;42:101086. PMC7559520.
Characterizing the adipocyte epigenome
Roh HC, Tsai LTY, Shao M, Tenen D, Shen Y, Kumari M, Lyubetskaya A, Jacobs C, Dawes B, Gupta RK, Rosen ED. Warming Induces Significant Reprogramming of Beige, but Not Brown, Adipocyte Cellular Identity. Cell Metab. 2018 May 1;27(5):1121-1137. PMCID: PMC5932137.
Roh HC, Tsai LT, Lyubetskaya A, Tenen D, Kumari M, Rosen ED Simultaneous transcriptional and epigenomic profiling from specific cell types within heterogeneous tissues in vivo. Cell Reports. 2017 Jan 24;18(4):1048-1061. PMCID: PMC5291126.
Roadmap Epigenomics Consortium*, Kundaje A, et al. Integrative analysis of 111 reference human epigenomes. Nature. 2015. Feb 19;518(7539):317-30. PMCID: PMC4530010.
Long JZ, Svensson KJ, Tsai L, Zeng X, Roh HC, Kong X, Rao RR, Lou J, Lokurkar I, Baur W, Castellot JJ Jr, Rosen ED, Spiegelman BM. A smooth muscle-like origin for beige adipocytes. Cell Metabolism. 2014 May 6;19(5):810-20. PMCID: PMC4052772
Ziller MJ, Gu H, Müller F, Donaghey J, Tsai LT, Kohlbacher O, De Jager PL, Rosen ED, Bennett DA, Bernstein BE, Gnirke A, Meissner A. Charting a dynamic DNA methylation landscape of the human genome. Nature. 2013 Aug 22;500(7463):477-81. PMCID: PMC3821869